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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Semin Cell Dev Biol. 2008 Oct 7;20(1):117–124. doi: 10.1016/j.semcdb.2008.09.007

Figure 4.

Figure 4

MoTP mediated larval melanocyte ablation and regeneration. (A) 3 dpf zebrafish larvae with normal or ontogenetically developed embryonic melanocytes. (B) Similar 3 dpf larvae exposed to melanotoxin MoTP from 30–72 hpf to kill melanocytes. Note faint pigmented detritus. (C) MoTP treated larvae after drug washout and melanocyte regeneration. (D) Schematic shows that wild-type zebrafish (black line) develop embryonic melanocytes prior to 60 hpf, and that between 60 hpf and 14 dpf, few new melanocytes are added to the larval pigment pattern. Treatment with MoTP following melanocyte differentiation (red line) results in rapid melanocyte death. Washout of MoTP results in regeneration of approximately 80% of embryonic melanocyte numbers. Treatment with MoTP before overt melanization (green line) kills developing embryonic melanocytes, but has no affect on melanocyte regeneration. The development of new melanocytes during metamorphosis (beginning at 14 days) is not affected by earlier MoTP treatments.