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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: J Pharmacol Exp Ther. 2008 Jun 12;326(3):829–837. doi: 10.1124/jpet.107.135798

Fig. 2.

Fig. 2

Hg2+ activates a signal transduction pathway that involves PKA and is independent of MLCK. Cells were treated with PKA inhibitor H89 and MLCK inhibitor ML-7 at time 0, and mercuric chloride was added after 60 min. A, cumulative mannitol flux as measured at 15-min intervals in presence of 30 μM H89 (n = 6). B, TEER as measured at 15-min intervals in presence of 30 μM H89 (n = 6). C, -fold change in raffinose permeability after addition of Hg2+ and its rescue by H89 at 1 μM concentration (n = 3). D, -fold change in mannitol permeability after addition of Hg2+ and its rescue by H89 at 1 μM concentration (n = 3). Cumulative mannitol flux as measured at 15-min intervals in presence of ML-7 (n = 6). D, TEER as measured at 15-min intervals in presence of ML-7 (n = 6).