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. Author manuscript; available in PMC: 2010 May 6.
Published in final edited form as: J Am Chem Soc. 2009 May 6;131(17):6149–6153. doi: 10.1021/ja807551e

Figure 4.

Figure 4

Iron-sulfur cluster transfer from native [2Fe-2S] SufA to apo Spore photoproduct lyase (SPL). ApoSPL (80 μM) was incubated with 10 molar excess of native SufA (0.4 iron and sulfide/monomer) in 0.1 M Tris-HCl pH: 8; 50 mM KCl, in the presence (A) or in the absence (B) of 5 mM DTT. The reaction was monitored by UV-vis. absorption spectroscopy before separation onto Ni-NTA column.