Fig. 1.

Effects of ovariectomy and estrogen treatment on AT₁ binding and AT_1B mRNA in the rat zona glomerulosa. AT₁ binding was measured by quantitative autoradiography. Consecutive sections were incubated with [¹²⁵I]Sar¹-Ang II in the presence or absence of losartan to determine binding to AT₁ receptors, and AT_1B mRNA was determined in additional consecutive sections by in situ hybridization, as described in Materials and Methods. E₂ indicates treatment with 17β-estradiol. Data are means ± SEM, n = 5–7 for each group. * p <0.05 compared to all other groups.