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Effects of ovariectomy and estrogen treatment on AT1 binding in the rat adrenal gland. Figures represent typical autoradiograms to show specific binding to AT1 receptors. E2 indicates treatment with 17β-estradiol. Sections were incubated with [125I]-Sar1-Ang II in the presence of PD 123319 to determine binding to AT2 receptors, as described in Materials and Methods. Arrows point to the adrenal zona glomerulosa, arrowheads to the adrenal medulla.
