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In vitro endothelial cell proliferation assay. Forty eight hours after transfecting VEGF plasmid into C2C12 cells, the VEGF containing medium was added for HUVEC cell incubation. Five days after incubation, the cell population was measured by a MTT assay. Data represents mean ± SEM (n = 3). Statistical analysis was done using one-way ANOVA and a Tukey's multiple comparison post-test. * p<0.05 is considered as statistical difference.
