Table 1.
Summary of reaction efficiency for minicircle DNA production. The amount of each plasmid population can be quantified from gel electrophoresis analysis (Quanti-One, Bio-Rad, Hercules, CA). The reaction efficiency was calculated using the equations in the Materials and Methods.
| Batch number | Precursor plasmid (%) | Backbone plasmid (%) | Minicircle DNA (%) | PhiC31-mediated recombination efficiency (%) | SceI-mediated digestion efficiency (%) | Overall efficiency (%) | ||
|---|---|---|---|---|---|---|---|---|
| Protocol I | LB 1:1 | 0116 | 39.9 | 7.6 | 52.5 | 89.9 | 97.1 | 87.3 |
| 0503 | 36.9 | 8.0 | 55.1 | 91.5 | 97.1 | 88.8 | ||
| Protocol II | LB 1:4 | 0503 | 29.9 | 7.8 | 62.3 | 94.3 | 97.5 | 92.0 |
| Protocol III | TB 1:1 | 0116 | 21.1 | 8.4 | 70.5 | 97.3 | 97.6 | 95.0 |
| 0503 | 17.4 | 8.8 | 73.7 | 98.4 | 97.6 | 96.1 | ||
| Protocol IV | TB 1:4 | 0503 | 13.2 | 2.6 | 84.1 | 98.0 | 99.4 | 97.4 |
| Protocol V | TB 1:8 | 0116 | 9.5 | 1.7 | 89.0 | 98.5 | 99.7 | 98.2 |
| 0503 | 11.5 | 4.3 | 84.2 | 98.7 | 99.0 | 97.7 | ||