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Specific lysis shown by representative triple (a), double (b), and single (c) peptide -reactive CTL clones generated from an HLA-B8+, healthy virus carrier SP after stimulation in vitro of PBMCs with the autologous LCL or HLA-A1/B8 homozygous BM LCL. Clones were tested in standard 4-h 51Cr-release assays against autologous or BM PHA blasts pulsed with RAK, RSK, RRK, FLR, or QAK peptide, or without added peptide (−). Effector/target ratio, 2:1.
