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Specific lysis shown by representative triple (■), double (○), and single (▴) peptide-reactive CTL clones generated from an HLA-B8+ healthy virus carrier SP after stimulation in vitro of PBMCs with the autologous LCL or HLA–A1/B8 homozygous BM LCL. Clones were tested in standard 4-h 51Cr-release assays against autologous PHA blasts pulsed with varying concentrations of RAK (a), RSK (b), or RRK (c) peptide. Effector/target ratio, 2:1.
