Figure 2.

Immunofluorescent detection of ENaC proteins in human primary mesothelial cells. These images are representative of 10 similar fields from 3 independent experiments. Primary human mesothelial cells grown on coverslips were incubated with specific antibodies against α, β, γ, and δ ENaC subunits. ENaC antibodies were detected (green channel) with Alexa Fluor 488 goat anti-rabbit IgG (heavy + light). Nonimmune IgG was used to as negative control (top left–most panel). Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (blue channel). Scale bars = 20 μm.