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Western blot of ENaC proteins in M9K Cells. Proteins were extracted from M9K cells. H441 cells were used as a positive control. Blots were incubated with specific antibodies against α, γ, and δ ENaC subunits (left panels). To demonstrate specificity, the blots were incubated with the ENaC antibodies in the presence of an excess of the corresponding immunizing peptides (right panels). These experiments were repeated three times with identical results.
