Figure 5.

CIH increased NADPH oxidase subunits, Nox4 and p22phox, levels in lung tissue. After 8 weeks of Control and CIH exposure, mice were killed, and lung tissue was homogenized. (A) Homogenized lung tissue was immunoprecipitated with antibodies directed against the p22phox subunit of the NADPH oxidase complex. Each bar represents the mean densitometric intensity expressed as percent control ± SEM from nine animals in each group. *P < 0.05 versus control. (B) Quantification of p22phox and 18S RNA was performed using cDNA amplification and real-time PCR. Each bar represents the mean copy number per 10⁹ copies 18S, expressed as percent control ± SEM from eight to nine animals in each group. *P < 0.05 versus Control. (C) Homogenized lung tissue was analyzed for the Nox4 subunit of NADPH oxidase complex. Each bar represents the mean densitometric intensity expressed as percent control ± SEM from five to six animals in each group. *P < 0.05 versus wild-type control. (D) RNA was extracted from lung tissue, and real-time PCR performed for Nox4 and 18S. Each bar represents the mean relative expression of Nox4/18S, expressed as percent control ± SEM from five animals in each group. *P < 0.05 versus Control.