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. Author manuscript; available in PMC: 2009 May 5.
Published in final edited form as: Ann Biomed Eng. 2008 Feb 6;36(4):661–671. doi: 10.1007/s10439-008-9445-8

FIGURE 5.

FIGURE 5

Effects of PMN tethering on melanoma cell adhesion to the EI monolayer. PMNs were treated with mAb to functionally block LFA-1 and Mac-1 (30 min, 4 °C); EI monolayer was treated with mAb against ICAM-1 (30 min, 4 °C); WM9 cells were treated with mAb against ICAM-1 (30 min, 4 °C). After each treatment, the excess of mAb was washed out by centrifuging cells down and re-suspending them in fresh medium. Cells were then injected into the parallel plate flow chamber for the adhesion assay. Blocking ICAM-1 on WM9 significantly reduced melanoma adhesion efficiency compared with both the control and anti-ICAM-1 on EI cases. *p < 0.05 compared with control samples. #p < 0.05 with respect to concurrent ICAM-1 blocking on the EI cells under the same shear condition. Values are mean ± SEM for N ≥ 3.