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. 2009 May 13;4(5):e5531. doi: 10.1371/journal.pone.0005531

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Southern hybridization demonstrating inactivation of secA2 and sigH. DraIII-digested DNA from each strain was probed for secA2, sigH and the hygR cassette used to inactivate sigH. Lane 1, BCG Tice; lane 2, BCGΔsecA2; lane 3, BCGΔsigH; lane 4, BCGΔsecA2ΔsigH (DDBCG); lane 5, a colony of BCGΔsecA2 with only one copy of sigH inactivated. secA2 contains two internal DraIII sites and DraIII-digested DNA was predicted to yield fragments of 307 bp, 785 bp, and 10,544 bp. sigH was predicted to be on a 2895 bp fragment. The hygR cassette contains an internal DraIII site and was predicted to yield 2938 bp and 1612 bp fragments after inactivation of sigH. (B) Differences in the localization and activity of SodA in BCG, DDBCG, and 3dBCG. A representative experiment shows the SOD units per ml of concentrated supernatant or lysate. (C) Immunoblot showing comparable amounts of SodA in lysates of DDBCG (lane 1) and 3dBCG (lane 2), despite the marked difference in SOD activity as shown in (b).