Figure 4. Inflammatory response genes are more up-regulated in influenza virus-infected P58^IPK−/− mice at 1 day post infection.

Gene Set Enrichment Analysis (GSEA) analysis was performed on a sample set derived from P58^IPK−/− and wild-type mice infected with the PR8 strain of influenza virus in triplicate at 1 day post infection. All infected samples were compared to genotype-matched mock-infected samples via microarrays analysis. Replicate samples were then in silico error-weighted pooled and re-ratioed to compare P58^IPK−/− gene expression to wild-type gene expression. Following GSEA analysis using only those genes which were significantly regulated, the top gene ontology categories related to the immune response were selected (see Figure S7 for entire table). An edge is placed between gene ontology categories if they share common genes, and the edge's thickness increases as the number of common genes increases. The log₁₀ ratio of P58^IPK to wild-type gene regulation is noted for selected gene ontology categories.