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. Author manuscript; available in PMC: 2009 Nov 15.
Published in final edited form as: Anal Chem. 2008 Oct 16;80(22):8649–8655. doi: 10.1021/ac801562f

Figure 5.

Figure 5

Detection of MMP-2 activity in tumor cell culture media. a) Representative emission spectra of QD655-MMP-2-Luc8 (220 nM) incubated in HT1080 cell culture media for 0, 2, or 17 h. b) QD655-MMP-2-Luc8 (250 nM) was incubated in HT29 cell culture media for 0, 2, or 17 h. c) The plot of the BRET ratio changes in HT1080 and HT29 cell culture media over time. d) Gelatin zymograph assay of the MMP-2 activity in cell media culturing HT1080 and HT29; commercial recombinant active MMP-2 was used as the control.