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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2009 Feb 7;236(2):194–201. doi: 10.1016/j.taap.2009.01.024

Figure 3. Acrolein-stimulated ROS is xanthine oxidase-dependent.

Figure 3

Serum-starved differentiated THP-1 cells were either left untreated (“none”) or were pre-incubated with allopurinol (100μM), apocynin (100μM), rotenone (100μM) or the calcium chelator BAPTA-AM (20μM) for 30 min prior to acrolein treatment. Relative rates of ROS production over 30min were measured and normalized to unexposed cells. (* p<0.05 vs “none”). B) Untreated cells or those pre-treated with allopurinol were variably exposed to acrolein for 30min as indicated. The cells were then lysed and xanthine oxidase activity was measured as described under Methods. Activity levels were normalized to untreated, unexposed (control) cells (* p<0.05 vs control; ** p<0.05 vs acrolein only). Each bar represents mean ± S.E. of 3 different measurements.