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. Author manuscript; available in PMC: 2009 Oct 28.
Published in final edited form as: Biochemistry. 2008 Oct 4;47(43):11332–11339. doi: 10.1021/bi801347a

Figure 3.

Figure 3

Crosslinking results with TM1 and TM2. A) Representative blots illustrating dimer and tetramer formation in the three double-Cys mutants that gave evidence of TM1-TM2 cross-linking. Electrophoresis was carried out for twice the normal time in the second panel to increase the separation between the 9+42 and 9-only bands. B) Summary of dimer and tetramer yields in the TM1/TM2 double-Cys mutants and the single-Cys controls. C) Two alternative models for the relative arrangement of the TM1 and TM2 segments in MotA. Dotted lines indicate the observed TM1-TM2 cross-links and solid lines indicate boundaries between subunits.