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. Author manuscript; available in PMC: 2009 Oct 28.
Published in final edited form as: Biochemistry. 2008 Oct 4;47(43):11332–11339. doi: 10.1021/bi801347a

Figure 4.

Figure 4

Inter-molecular crosslinking between positions in TM1 and TM3. A) Representative gels showing results with the 3/178 and 9/181 mutants, illustrating the method of band assignment by comparison with single-Cys mutants. Double mutants were oxidized using iodine at room temperature (approximately 20°); single mutants were oxidized at a higher temperature (54°) to increase yield. B) Additional examples of TM1-TM3 crosslinking. The corresponding single-Cys mutants (not shown) gave results similar to those in panel A, with TM1-TM1 products at the lowest position and TM3-TM3 products at the highest position. C) Summary of yields of TM1-TM3 cross-linking (n.d., none detected).