Figure 6. CAV-2 and CAR colocalise in axonal endosomes.

(A) CAR colocalises with CAV-2 and TeNT H_C in MNs. Acid-washed MNs incubated with CAV-Cy3 and AlexaFluor488-TeNT H_C for 45 minutes show internal virions in compartments containing CAR and TeNT H_C (arrowheads) in MN axons. Quantification is shown on the right panel (3 independent experiments, 95 carriers in total; error bars represent 7.3%). (B) Magnetic pull-down of TeNT H_C-containing vesicles. MNs were incubated with TeNT H_C coupled to superparamagnetic Fe beads for 60 minutes and then lysed in absence of detergent (see Materials and Methods). A representative experiment (left panel) and its quantification (right panel, 3 independent experiments) are shown. Western blot analysis after magnetic purification shows a specific association of CAR with TeNT H_C transport carriers. Scale bars = 10 µm.