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Fluorescent microscope images of SKBR3 human breast cancer cells after pretargeting trastuzumab antibodies modified with rhodamine and norbornene and subsequent labeling with 3. (A) Rhodamine channel. (B) Near-IR channel (tetrazine-VT680). (C) Merged image of A and B. Images were also taken of cell surfaces targeted with a control antibody modified with only rhodamine and exposed subsequently to 3. (D) Rhodamine channel. (E) Near-IR channel.
