Figure 1.

Cocaine regulates acetylation of histone H3 (acH3) and trimethylation of Lys9 of H3 (3mK9H3) on NFκB gene promoters (tissue was collected 4–6 h after cocaine administration). A, Increased binding of acH3 at the p105/p50, p65/Rel-A, and IκBβ promoters (*p < 0.05, Student's t test, n = 6–8), and no significant changes on the IκBα or c-rel promoters. B, A general decrease in 3meK9H3 binding, a repressive mark, on all NFκB promoters examined (*p < 0.05, Student's t test, n = 6–8). In contrast, C shows no changes in 3mK4H3 binding at any of the NFκB promoters. All data are expressed as mean fold change ± SEM and are normalized to total input DNA.