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. 2009 May 14;4(5):e5542. doi: 10.1371/journal.pone.0005542

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Prieur et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Coomassie staining of the gel showing expression of recombinant GST-fusion proteins purified from bacteria, asterix for full length recombinant GST-E2F1 and GST. (B) GST pull-down assay of in vitro translated unsumoylated (DRIL1) or sumoylated (DRIL1-S) DRIL1, and Luciferase control, with GST-E2F1 and GST control. (C) Quantification by ImageJ software of DRIL1 species recovered by pulldown vs input (% of input band intensity).