Table 1. microRNA expression platforms and experimental procedures.
Code | Protocol | Platform | miRBase release | # of miRNAs | RNA (ug) | small RNA enrichment | Labeling Kit | Dye | Agitation | # of replicates |
AGL | one color | Human miRNA V2 Oligo Microarray | 10.1 | 723 | 0.1 | no | Agilent | Cy3 | yes | 3 |
AMB | one color | mirVana miRNA Bioarray V9.2 | 9.2 | 312 | 20 | yes | Ambion | Cy5 | no | 3 |
EXQ | one color | miRCURY LNA microRNA Array v.10.0 | 10.0 | 704 | 1 | no | Exiqon | Hy5 | no | 3 |
IVG | two color | Ncode Human miRNA Microarray V3 | 10.0 | 699 | 5 | no | Invitrogen | Alexa5 Alexa3 | no | 3×2 |
TRY | one color | 3D-Gene Human miRNA oligo chip | 10.1 | 723 | 0.5 | no | Exiqon | Hy5 | yes | 3 |