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Matrix sequence-discrimination assay for three-zinc finger peptides generated using three regions of the HIV-1 LTR. 0, 5, and 20 nM of each purified peptide (HIV A, D, and F) were tested against each DNA-binding site (DNA: A, D and F). The letter “f” denotes free DNA and “b” denotes protein-bound complex. DNA binding by each protein was only observed to the sequences against which the zinc fingers had been designed.
