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. Author manuscript; available in PMC: 2009 May 6.

Published in final edited form as: J Cell Physiol. 2008 Oct;217(1):127–137. doi: 10.1002/jcp.21483

Sfrp2 modulation of beta-catenin in vitro and in vivo. (A) Transfection assay using the Top/Fop-flash luciferase reporter system. Wnt1and to a lesser extent, Wnt4 and Wnt9a activated the Top-flash reporter gene, demonstrating that they can signal via the canonical pathway and co-transfection with Sfrp2 inhibited such activation. Wnt5a and Wnt5b had no effect on the reporter gene, suggesting they are not signaling through the canonical pathway in our experimental system. (B) Sections of interphalangeal joint at E15.5 stained with a beta-catenin specific antibody. Note subtle increased accumulation of beta-catenin in the epiphyseal region (arrow) of the Sfrp2-/- mouse (right panel). (C) Sections of first phalanx at E17.5 stained with the same antibody. Notice the increase beta-catenin localization in the epiphyseal area (arrow) in the mutant mouse versus the WT. (D) Sections of first phalangeal elements of TOPGAL/Sfrp2 trangenic mice, showing increased β-galactosidase staining in the articular chondrocytes of some interphalangeal joints of the Sfrp2 null mice (arrow).

Sfrp2 modulation of beta-catenin in vitro and in vivo. (A) Transfection assay using the Top/Fop-flash luciferase reporter system. Wnt1and to a lesser extent, Wnt4 and Wnt9a activated the Top-flash reporter gene, demonstrating that they can signal via the canonical pathway and co-transfection with Sfrp2 inhibited such activation. Wnt5a and Wnt5b had no effect on the reporter gene, suggesting they are not signaling through the canonical pathway in our experimental system. (B) Sections of interphalangeal joint at E15.5 stained with a beta-catenin specific antibody. Note subtle increased accumulation of beta-catenin in the epiphyseal region (arrow) of the Sfrp2-/- mouse (right panel). (C) Sections of first phalanx at E17.5 stained with the same antibody. Notice the increase beta-catenin localization in the epiphyseal area (arrow) in the mutant mouse versus the WT. (D) Sections of first phalangeal elements of TOPGAL/Sfrp2 trangenic mice, showing increased β-galactosidase staining in the articular chondrocytes of some interphalangeal joints of the Sfrp2 null mice (arrow).