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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Microcirculation. 2009 May;16(4):289–306. doi: 10.1080/10739680902801949

Figure 6. Extracellular Insulin Receptor Cleavage and Insulin Resistance.

Figure 6

Panel A: Typical micrographs of immunolabel (Vector NovaRed) for the extracellular domain binding site of the insulin receptor α on fresh leukocytes (neutrophils and monocytes) from WKY and SHR. Note the reduced density of the insulin binding sites on the SHR leukocytes associated with about 20% average reduction of the receptor density on the plasma membrane and with reduced transport of glucose (shown with fluorescence-tagged analog of glucose in panel B) into the cell cytoplasm (25). Panel C: Glucose transport into naïve leukocytes from the normotensive Wistar strain before and after a 30 min incubation in fresh plasma of the Wistar (Wistar and Wistar-W in panel C) and in plasma from WKY and SHR (Wistar-WKY and Wistar-SHR in panel C). For quantitative measurements see (25).