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. Author manuscript; available in PMC: 2009 May 6.
Published in final edited form as: Cell Physiol Biochem. 2008 Dec 9;22(5-6):745–756. doi: 10.1159/000185558

Fig.3.

Fig.3

Effects of the p53 and NFκB binding sites on mdr1b promoter activity shown by mutation constructs.

A. Point mutation of mdr1b sequence -476/+45 was made at either the NFκB binding site or the p53 binding site. These sequences were cloned into the pGL3.0 luciferase reporter vector.

B. Luciferase assays were performed 30 h after RBE4 cells were transfected with the mutant or wildtype plamids, with (solid bar) or without (empty bar) TNF treatment for 6 h immediately before cell lysis. Mutation of the NFκB binding site nearly completely abolished the promoter activity, and the cells no longer responded to TNF by an increase of luciferase intensity. Mutation of the p53 binding site had no effect.