Table 2. Cloning primers for the wildtype and mutant rat mdr 1 promoter sequences.

Name	Sequence
-963/+45 forward	5′-agcgcgctagccccgtggaccatgagttaag
-476/+45 and -476/+607 forward	5′-gcgaagctagcgccagaaaaccgaatggata
-250/+45 forward	5′-gcatggctagcaaccgtgcactatccaggta
-218/+45 forward	5′-gacctgctagcccatatggagagttacctgaaca
-185/+45 forward	5′-gccatgctagctctgtgttaatgtctggggaat
-159/+45 forward	5′-ctcaggctagcgctcccttctcaaaaactcaga
-12/+607 forward	5′-gcgaagctagcgccagaaaaccgaatggata
-476/+607 and +12/+607 reverse	5′-gcaagctcgagaggccctcttcaaactccat
963/+45, -476/+45, -250/+45, -218/+45, -185/+45 and -159/+45 reverse	5′-atcagctcgagggcctcagcctcttacagc
-476/+45 NFκB mutant forward	5′-catgtctgtgttaatgtctgctcaattccagctc
-476/+45 NFκB mutant reverse	5′-cagacattaacacagacatgtctctacatg
-476/+45 p53 mutant forward	5′-catatggagagttacctgaatcggtagagacatg
-476/+45 p53 mutant reverse	5′-ttcaggtaactctccatatggaggtgtata

Restriction enzyme sites were included in the 5′ ends of the primers for directional cloning of PCR products. NheI sites are single underlined and XhoI sites are double underlined. In the primers designed for site-directed mutation, the mutated sites are in bold and underlined with dotted lines.