Fig. 3.

Pathogenic anti-DNA antibody synergizes with HMGB1 in the induction of proinflammatory responses in kidney cells. MRL/lpr MCs were incubated with HMGB1 (10 µg/ml) or mAb (50 µg/ml), alone (A) or together (B), for 6 h before gene expression was measured by real-time PCR. The gene for Cyclin I (CCNI) was used in A as a control. C, BALB/c mice were injected with 10 µg of HMGB1 intraperitoneally and/or 300 µg of mAb intravenously, 24 h before the kidneys were removed (4 mice per group). Gene induction in the treated cells (A, B) or kidneys (C) was analyzed by real-time PCR. Mean fold changes and SD are shown. D, Serum CXCL1 was measured by ELISA in mice injected with antibody, HMGB1, or both. Values shown represent the mean and SD.