Table 1.

Metal content of GLX2−1 analogues

	Fe (eq)	Zn(II) (eq)
Wild-type GLX2−1
No added metal	0.3±0.2	0.3±0.1
250 μM Fe plus Zn(II) added in the medium	0.3±0.1	0.3±0.2
3-fold excess of Fe added after purification	1.9±0.1	0.2±0.1
3-fold excess of Zn(II) added after purification	∼0.1	1.6±0.2
3-fold excess of Fe plus Zn(II) added after purification	1.6±0.2	1.6±0.2
GLX2−1 R246L
250 μM Fe plus Zn(II) added in the medium	∼0.1	0.6±0.1
3-fold excess of Fe added after purification	2.4±0.5	0.5±0.2
3-fold excess of Zn(II) added after purification	0.1	1.4±0.2