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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Exp Cell Res. 2009 Feb 3;315(7):1234–1246. doi: 10.1016/j.yexcr.2009.01.021

Figure 2. Intracellular colocalization of cav-1 with gelatin and collagen IV degradation products in HUVEC.

Figure 2

Equal numbers of HUVEC transfected with cav-1-mRFP construct were grown for 16 h on glass coverslips coated with either gelatin containing 25 μg/ml DQ-gelatin (A) or rBM (Cultrex) containing 25 μg/ml DQ-collagen IV (B). After 16 h, confocal images were taken of live cells (DIC), cav-1-mRFP (red) and DQ-substrate degradation products (green). DQ-substrate degradation products (green) are present intracellularly in HUVEC grown on gelatin and both intracellularly and pericellularly in HUVEC grown on rBM (see inserts). Colocalization of cav-1-mRFP and DQ-substrate degradation products appears yellow in the merged images. N, nucleus. Bar, 20 μm.