Fig. 4.

Insulin-stimulated increase in 2-deoxyglucose uptake in L6 cells was blocked by MARCKS siRNA. a L6 cells were grown to 80% confluence and allowed to fuse into myotubes for 4 days. Cells were transfected with siRNA for MARCKS purchased from Ambion as described. Forty-eight hours later, cells were treated with insulin for 30 min. Half of the cells were lysed to perform western blot analysis (a), in which enolase was used to access loading of the gel and half were assayed for 2-deoxy-D-[1,2-³H]glucose uptake (b). The uptake assay was performed in triplicate on two occasions with similar results. Dark grey bars show basal uptake and light grey bars show insulin-stimulated uptake. Results are mean±SEM. *p<0.05 compared with scrambled siRNA for insulin response, Student’s t test