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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Protein Expr Purif. 2008 Oct 19;64(1):82–88. doi: 10.1016/j.pep.2008.10.005

Fig. 3.

Fig. 3

Relationship between the PDE8A1 concentration and cAMP conversion. The assays were carried out at a protein concentration range of 5.3 to 84.8 ng/ml and in a buffer of 20 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 1 mM DTT, 3H-cAMP (20000 cpm/assay) at 24°C for 15 min.