Figure 7.

Distribution of the G6-G7 roll. The distribution of G6-G7 roll, which is important for intercalation of Phe37 of HMGB1a between the G6-G7 base-pair step is plotted for the AGGC and the TGGA sequence contexts. The distributions of CP-, OX- and undamaged DNA are plotted in each sequence context. The distribution for undamaged DNA is plotted as a solid line, the distribution for CP-DNA is plotted as a dashed line and that for OX-DNA is plotted as a dotted line. The bend angle of DNA in the crystal structure of CP-DNA bound to HMGB1a (21) is plotted as a vertical dashed line. The region of roll ±10° of the roll in the crystal structure of CP-DNA bound to HMGB1a is plotted alongside to highlight differences between CP- and OX-DNA in the TGGA sequence context.