Table 2.
MSE for residuals calculated as the difference (in degrees Celsius) between experimental melting temperatures and predictions obtained by fractional programming without salt correction (21) and with salt corrections performed using the SantaLucia model equation (1)
| Salt Conc. | Primer length 9–30 | Primer length 20–25 | ||||
|---|---|---|---|---|---|---|
| (M) | GC content 8%–80% |
GC content 25%–75% |
||||
| Number of duplexes | MSE w/o salt correction | MSE with salt correction | Number of duplexes | MSE w/o salt correction | MSE with salt correction | |
| 0.069–0.15 | 351 | 150.03 | 2.30 | 158 | 148.91 | 2.25 |
| 0.22 | 152 | 47.44 | 2.71 | 72 | 43.14 | 3.18 |
| 0.62–0.621 | 152 | 8.98 | 2.52 | 72 | 6.90 | 1.38 |
| 1–1.02 | 157 | 4.75 | 4.97 | 74 | 2.61 | 2.76 |