Table 3.

Average and standard deviation for the difference (in degrees Celsius) between experimental melting temperature and predictions made by the SantaLucia model equation (1) on duplexes with one and two mismatches

Number of mismatches	Length range	GC content range	Number of duplexes	Average difference	Standard deviation
1	9–16	21%–78%	110	0.56	2.06
2	12–14	50%–75%	28	−1.25	2.70