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. 2009 Mar 5;37(8):2493–2503. doi: 10.1093/nar/gkp079

Figure 1.

Figure 1.

Multiple sequence alignment of the DNA-recognition helix within candidate proteins from the CRP/FNR family of transcription factors. Residues in CRP known to make specific contacts within DNA bases (22) are highlighted in black. The specificity-determining residues from Rodionov and coworkers (20) correspond to columns 4, 5 and 10. Note that the specificity determining residues for PrfA are somewhat ambiguous due to an insertion in the alignment relative to CRPwt. The numbers in parentheses denote the region of the protein shown in the alignment and the mutant column designates the corresponding CRP mutation used in this study.