Figure 4.

Expression of CKT2 in testicular cells. (A) Immunohistochemistry of testis section with anti-CKT2 antibody; nuclei of spermatogonia (arrow) and spermatids (asterisks). (B) Testis incubated with nonimmune IgG antibody. (C) Immuno-gold labeling for CKT2 of a spermatid at step 13 of differentiation showing that CKT2 is present in the nuclei of spermatids undergoing chromatin condensation. As shown in the magnified views, the labelling is weaker in the more condensed apical nuclear region (D), as compared to the stronger labelling in the less condensed posterior nuclear region (E) (see the labelling pattern of spermatids at different steps of differentiation in Supplementary Figure 3). Data shown are representative of two independent experiments.