Figure 3.

Nucleosome eviction does not correlate with chromatin modification, BRG1 recruitment or H2A.Z deposition. The levels of H4Ac, H3Ac, H3K4Me3, BRG1 recruitment and H2A.Z deposition at the HLA-DRA promoter were analyzed by qChIP in wild-type Raji B cells (WT), CIITA-deficient B cells (CIITA^−/−) and RFX-deficient B cells (RFX^−/−). Results were corrected for nucleosome density using antibodies directed against unmodified histone H3, and were normalized relative to a control position at the TBP promoter. Results are expressed relative to the values observed in wild-type cells, and represent the means and standard deviations derived from three independent experiments. Primers used are indicated in Supplementary Table 1.