Figure 3.

HapR regulates expression from promoters containing either Motif 1 or Motif 2. Promoter-lux fusions were constructed and transformed into WT and hapR-deleted strains. Fold induction by HapR was determined as the ratio between bioluminescence readings from the reporter fusions in WT/ΔhapR. Negative fold induction indicates repression. Each bar represents three data points, and error bars represent standard deviations. Divergently transcribed genes with HapR binding sites located in between are indicated with boxes.