Figure 4.

Cisplatin treatment decreases DNMT3b mRNA stability and steady-state levels. (A) Two days after siRNA transfection, cells were treated with 50 μM cisplatin for 8 h, RNA was isolated and RT–qPCR performed (shown as the mean ± SEM from three experiments; *P ≤ 0.05); the data were normalized to GAPDH. (B) The half-lives of DNMT3b variant 3 and housekeeping GAPDH mRNAs in RKO cells transfected with C siRNA (see Supplementary Figure 1 for HuR siRNA cells), either untreated or treated with cisplatin (50 μM), were quantified by using RT–qPCR and calculated as described in the legend of Figure 3B; data represent the mean ± SEM from three independent experiments.