Figure 8.

HuR silencing and cisplatin treatment affect global DNA methylation and the methylation of specific DNMT3 target DNA sequences. Two days after siRNA transfections, cells were treated with 25 μM cisplatin for 24 h and the DNA was extracted to measure (A) global DNA methylation by HPLC coupled with MS (*P ≤ 0.05; **P ≤ 0.01) and (B) the DNA methylation status of the repetitive sequences D4Z4 and Sat2 by bisulfite sequencing. Black and white squares represent methylated or unmethylated CpGs, respectively (*P ≤ 0.05; **P ≤ 0.01).