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(a) Fluorescence emission spectra of TO-GMP (65 nM) in the presence of varying concentrations of GTP Class I (0, 0.0195, 0.039, 0.078, 0.1563, 0.625 and 2.5 µM, frssom bottom to top). The insert shows for maximum fluorescence versus aptamer concentration curve fitting (Kd = 60 nM). (b) Dependence of the fluorescence on the concentration of GTP Class I aptamer for: 1; TO; 2 and TO-C10 (all measurements at 50 nM of dyes).
