Skip to main content
. 2009 Mar 30;37(8):e64. doi: 10.1093/nar/gkp184

Figure 1.

Figure 1.

Schematic representation of the mRNA display selection of scFv on a microfluidic chip. Step (1): an scFv DNA library is transcribed to mRNA in vitro. Step (2): the mRNA library is ligated with a PEG-puromycin spacer. Step (3): the RNA-PEG-puromycin library is translated in a cell-free translation system. Step (4): the mRNA-displayed scFv library is injected into a sensor chip on which a target antigen is immobilized. Step (5): the bound scFv is eluted competitively with the free antigen. Step (6): the RNA is amplified by RT-PCR and used for the next round of selection or cloning and sequencing.