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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Jan 27;1788(5):1033–1043. doi: 10.1016/j.bbamem.2009.01.006

Figure 2.

Figure 2

Fourth cycle compression isotherms of 800 μg Survanta on a saline buffered subphase containing albumin (2 mg/mL when present) and the stated chitosan concentrations. (a) □ Survanta; ○ Survanta-albumin; ▷ Survanta-albumin with 0.005 mg/mL chitosan, ◁ Survanta-albumin with 0.001 mg/mL chitosan; △ Survanta-albumin with 0.0005 mg/mL chitosan; ⬠ Survanta-albumin with 0.0001 mg/mL chitosan. In this concentration regime, increasing chitosan concentration yields increasing surfactant adsorption. From Table 1, charge neutralization of the Survanta and albumin is reached between 0.0005–0.005 mg/mL chitosan. Note that for .001 mg/ml chitosan, more Survanta adsorbs (isotherm shifted to larger trough areas) that the control Survanta on a clean subphase.

(b) □ Survanta; ○ Survanta-albumin; △ Survanta-albumin-chitosan 0.5 mg/mL, ▽ Survanta-albumin-chitosan 0.1 mg/mL; ◁ Survanta-albumin-chitosan 0.01 mg/mL; ▷ Survanta-albumin-chitosan 0.005 mg/mL. For chitosan concentrations greater than that necessary for charge neutralization (Table 1), surfactant adsorption decreased. The shaded area denotes the trough area over which the surface pressure was averaged for each chitosan concentration to obtain the surfactant relative adsorption plotted in Fig. 6.