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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Jan 27;1788(5):1033–1043. doi: 10.1016/j.bbamem.2009.01.006

Figure 5.

Figure 5

Fluorescence images of 800 μg Survanta spread on a saline buffered subphase containing 2 mg/mL albumin and 0.5 mg/mL chitosan. Images are 1023 μm by 789 μm.

First Cycle (a) Π = 25 mN/m during compression. The black homogenous background dominates the interface as the albumin prevents the Survanta from spreading as a monolayer. (b) Π = 18 mN/m during expansion. Survanta breaks through the interface; extended (>1000 μm) immiscible Survanta (mottled gray) and albumin (black) domains coexist on the interface.

Second Cycle (c) Π = 21 mN/m during compression. (d) Π = 18 mN/m during expansion. The albumin and Survanta domains coexist during the compression and expansion cycle.

Third Cycle (e) Π = 21 mN/m during compression. (f) Π = 18 mN/m during expansion. Albumin and Survanta domains coexist on the interface.

Fourth Cycle (g) Π = 24 mN/m during compression. (h) Π = 18 mN/m during expansion. Albumin remains on the interface through the fourth compression and expansion cycle. Apparently a sufficient quantity of Survanta does not adsorb to completely expel the albumin from the interface.