. Author manuscript; available in PMC: 2009 Oct 3.

Published in final edited form as: Anal Chim Acta. 2008 Jun 5;627(1):117–128. doi: 10.1016/j.aca.2008.05.074

Table 4.

Summary of mono- (Mo) and bifunctional (Bi) adducts obtained by treating dimeric human gluthatione S-transferase (GST) with either PDG or BDG, followed by digestion with trypsin (T) or chymotrypsin (C). Indexes (a) and (b) indicate cognate subunits. Monoisotopic masses were calculated from the incremental masses described in Scheme 2 and from theoretical peptide masses provided by the PeptideMass calculator [67].

Reagent	Obs. mass (Da)	Calc. mass (Da)	Digestion product	Modified site(s)
PDG	2809.4776	2809.4880	73–78(a)/63–79(b) + Bi, C	R74(a) ↔ R74(b)
PDG	860.4357	860.4396	184–189 + Mo, C	R186
BDG	2809.4776	2809.4819	9–17(a)/8–21(b) + Bi, C	R13(a) ↔ R13(b)
	2300.2505	2300.2400	73–78/9–21 + Bi, C	R74 ↔ R13 or R74 ↔ R11
	1646.8526	1646.8571	177–189 + Bi, C	R182 ↔ R186
	1815.9114	1815.8984	1–13 + Mo, T	R11
	1778.8734	1778.8649	18–19/63–72 + Bi, C	R18 ↔ R70