Skip to main content
. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Exp Hematol. 2008 Oct 25;37(1):19–30. doi: 10.1016/j.exphem.2008.08.008

Figure 6.

Figure 6

Wnt3a blocks VCAM-1 expression on a HSC enriched fraction. (A) VCAM-1 gene expression (by semi-quantitative RT-PCR) is shown normalized to actin for freshly isolated HSC enriched cells from bone marrow, the same hematopoietic cells cultured overnight on vector control stroma or on Wnt3a secreting stroma. Also shown are the corresponding PCR products at 28 and 31 cycles for β-actin and 35 and 38 cycles for VCAM-1. (B) Stem cell enriched LSK were transduced with vector control or Wnt3a containing IRES-GFP retroviruses and cultured stromal cell-free with cytokines. Shown are the flow cytometry analyses of VCAM-1 staining in cells gated for HSC rich Lin- Sca-1Hi c-Kit Hi fraction after 5 days of culture. The grey filled curve illustrates LSK transduced with Wnt3a, while the black line shows LSK transduced with the control vector. (C) Averaged MFI (VCAM-1) are shown for the LSK represented in (B). Shown is one representative experiment of three that produced similar results. Error bars indicate standard error and asterisks indicate statistical significance, *** (p<0.0001).