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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Exp Hematol. 2008 Oct 25;37(1):19–30. doi: 10.1016/j.exphem.2008.08.008

Figure 7.

Figure 7

Wnt3a preserves the ability of hematopoietic cells to engraft and differentiate even after 5 days of co-culture. (A) A schematic of the experimental design is given. (B) CD45.2+ cells were recovered from the engrafted tissues and identified by gating one month after transplantation. Yields per transplanted cell per organs (two femurs) were calculated. (C) Flow cytometry contour plots show engraftment and differentiation from Wnt3a co-culture cells in bone marrow. CD19 and CD11b versus Gr-1 discern B and myeloid lineages (two middle panels), while the right panel shows cells with stem cell-like LSK characteristics. (D) The VCAM-1 levels on donor (CD45.2+) and host (CD45.1+) cells recovered from bone marrow and gated on the stem cell rich LSK fraction are shown. (E) Donor type B lineage and myeloid cells in spleen and (F) lymphocytes in the thymus were characterized according to expression of CD4 and CD8 surface proteins. The far right panel in (F) was gated on CD4- CD8- cells and shows progression through CD25+ CD44- DN3 and CD25- CD44- DN4 thymic subsets. Shown is one representative experiment of two that produced similar results, using 3 mice per transplant group. Error bars indicate standard error and asterisks indicate statistical significance, * (p<0.05), *** (p<0.0001).